A vacuum glove box, also known as a vacuum inert gas operation box, allows experimental samples to be safely placed in and taken out, enabling free operation, reaction, and testing under oxygen-free and water-free conditions. It can assist research institutions in conducting experiments with low-energy radioactive substances, performing sterile operations for microbial inoculation, and carrying out qualitative and quantitative analysis of chemical substances, among other sealed isolation operations, to protect human tissues from damage caused by electrical radiation.